Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin

Sally P Wheatley

doi:10.1007/978-1-0716-1904-9_7

Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin

Methods Mol Biol. 2022:2415:95-103. doi: 10.1007/978-1-0716-1904-9_7.

Author

Sally P Wheatley¹

Affiliation

¹ School of Life Sciences, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, UK. sally.wheatley@nottingham.ac.uk.

PMID: 34972948
DOI: 10.1007/978-1-0716-1904-9_7

Abstract

This paper describes an easy method to enrich the harvest of adherent mammalian cells at each stage of mitosis (from prometaphase to cytokinesis) by combining Eg5 inhibition using dimethylenastron (DMA) with mitotic shake-off, followed by timed release from the drug.

Keywords: Dimethylenastron (DMA); Eg5; Mammalian; Mitotic synchrony; Survivin.

MeSH terms

Animals
Cytokinesis*
HeLa Cells
Humans
Mammals
Mitosis*
Prometaphase
Survivin

Substances

Survivin