Preparation and Fluorescent Labeling of Cell-Derived Micelles and Profiling on Glycan Microarrays

Abstract

Mammalian cell surface lectins mediate many important biological interactions which regulate physiological processes and therefore profiling mammalian cells on glycan microarray is of interest. However, many whole mammalian cells are not compatible with glycomics microarray formats and instead cell-derived micelles are prepared and profiled instead of whole cells as they can accurately represent the parental cell glycome. In this chapter, we describe the preparation of cell-derived micelles from mammalian cells, their labeling using a membrane-incorporating dye, and their profiling on a glycan microarray platform.

Keywords: Mammalian cells; Membrane labeling, cell surface glycosylation, glycan microarray; Micelles; Vesicles.