Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species

Jacqueline de Oliveira Zoccolotti; Alberto José Cavalheiro; Camilla Olga Tasso; Beatriz Ribeiro Ribas; Túlio Morandin Ferrisse; Janaina Habib Jorge

doi:10.1371/journal.pone.0261884

Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species

PLoS One. 2021 Dec 31;16(12):e0261884. doi: 10.1371/journal.pone.0261884. eCollection 2021.

Authors

Jacqueline de Oliveira Zoccolotti¹, Alberto José Cavalheiro², Camilla Olga Tasso¹, Beatriz Ribeiro Ribas¹, Túlio Morandin Ferrisse¹, Janaina Habib Jorge¹

Affiliations

¹ Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil.
² Department of Biochemistry and Organic Chemistry, Institute of Chemistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil.

Abstract

This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Infective Agents
Biofilms
Candida albicans*
Cryptocarya*

Substances

Anti-Infective Agents

Grants and funding

The author Jacqueline de Oliveira Zoccolotti received a doctoral scholarship by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 88887.513643/2020-00. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.