Effects of H3PO4 modified biochar on heavy metal mobility and resistance genes removal during swine manure composting

Zhiqiang Chen; Huanyu Bao; Qinxue Wen; Yiqi Wu; Qiqi Fu

doi:10.1016/j.biortech.2021.126632

Effects of H₃PO₄ modified biochar on heavy metal mobility and resistance genes removal during swine manure composting

Bioresour Technol. 2022 Feb:346:126632. doi: 10.1016/j.biortech.2021.126632. Epub 2021 Dec 28.

Authors

Zhiqiang Chen¹, Huanyu Bao¹, Qinxue Wen², Yiqi Wu³, Qiqi Fu¹

Affiliations

¹ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China; School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.
² State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China; School of Environment, Harbin Institute of Technology, Harbin 150090, PR China. Electronic address: wqxshelly@163.com.
³ Research Institute of Standards and Norms, Ministry of Housing and Urban-Rural Development, Beijing 100835, PR China.

PMID: 34971775
DOI: 10.1016/j.biortech.2021.126632

Abstract

In this research, static composting treatments of swine manure with forced ventilation were conducted to study the effects of biochar (BC) and H₃PO₄ modified biochar (BP) addition on heavy metals (HMs) stabilization, profiles of antibiotic resistance genes (ARGs), heavy metals resistance genes (MRGs) and bacterial communities during swine manure composting. After 42 days of the composting, compared to control (CK), BC and BP decreased the concentration of diethylenetriamine pentaacetic acid extractable Cu and Zn by 12.04%, 15.15% and 26.91%, 36.50%, respectively. Furthermore, BC and BP treatments reduced the total abundances of nine ARGs by 4.02% and 66.21%, and five MRGs by 53.66% and 58.81%, compared to CK in the compost product. Network analysis and square structural equation model analysis revealed that the decrease of ARGs and MRGs in BP treatment was related tothe change in bacterial community during the composting, rather than differences in co-selection pressure.

Keywords: Antibiotic resistance genes (ARGs); Composting; Heavy metals; Modified biochar; Swine manure.

MeSH terms

Animals
Anti-Bacterial Agents
Charcoal
Composting*
Genes, Bacterial
Manure
Metals, Heavy*
Swine

Substances

Anti-Bacterial Agents
Manure
Metals, Heavy
biochar
Charcoal