To study the effect of abnormal intestinal flora on the risk of colorectal cancer and the effect of heparin on the formation of bacterial biofilm, 50 patients with colorectal cancer and 50 healthy subjects were selected. The distribution and quantity of bacteria in feces, the levels of D-lactic acid and endotoxin in serum of the two groups were detected. Intestinal flora strains and biofilm growth were also detected in patients with colorectal cancer cultured in different heparin concentrations (0 mg/mL, 5 mg /mL, 10 mg/mL, and 20 mg/mL). The results showed that there was significant difference in the number of major strains of intestinal flora between healthy subjects and colorectal cancer patients before and after operation (P < 0.05). The serum D-lactic acid levels (1.41 ± 0.39, 6.38 ± 1.42 μg/mL) and endotoxin levels (0.62 ± 0.09, 0.80 ± 0.15 EU/mL) in the experimental group were higher than those in the control group (0.91 ± 0.52 μg/mL) (0.05 ± 0.02 EU/mL) before and after operation (P < 0.05). The amount of biofilm formation increased significantly with the increase of heparin concentration (P < 0.05). In summary, there was a close relationship between the occurrence of colorectal cancer and abnormal intestinal flora. Heparin may have a positive effect on regulating intestinal flora in patients with colorectal cancer, which provided certain reference value for the treatment of colorectal cancer.
Keywords: Intestinal cancer; biofilm; heparin; intestinal flora.