Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials

Abstract

Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method. The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on. The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm. With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased. The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS. The mechanical strength of BCA was also significantly enhanced, and the mineralization ability of bioglass was effectively deployed with the adding SA of BCA. The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA. It indicates that BCA composite scaffolds have potential applications in bone issues engineering.

Keywords: Biological activity; Bone tissue engineering; Composite scaffolds; Cross-linking; Freeze-drying method.