DNA Origami-Encoded Integration of Heterostructures

Xinpei Dai; Xiaoliang Chen; Xinxin Jing; Yinan Zhang; Muchen Pan; Mingqiang Li; Qian Li; Pi Liu; Chunhai Fan; Xiaoguo Liu

doi:10.1002/anie.202114190

DNA Origami-Encoded Integration of Heterostructures

Angew Chem Int Ed Engl. 2022 Mar 7;61(11):e202114190. doi: 10.1002/anie.202114190. Epub 2022 Jan 27.

Authors

Xinpei Dai^{1

2

3}, Xiaoliang Chen⁴, Xinxin Jing^{4

5}, Yinan Zhang⁴, Muchen Pan⁴, Mingqiang Li⁴, Qian Li⁴, Pi Liu⁶, Chunhai Fan⁴, Xiaoguo Liu⁴

Affiliations

¹ Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China.
⁴ School of Chemistry and Chemical Engineering, Frontiers Science Centre for Transformative Molecules, Shanghai Jiao Tong University, Shanghai, 200240, China.
⁵ Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acids Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
⁶ Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Biodesign center. 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China.

PMID: 34962699
DOI: 10.1002/anie.202114190

Abstract

Integrating dissimilar materials at the nanoscale is crucial for modern electronics and optoelectronics. The structural DNA nanotechnology provides a universal platform for precision assembly of materials; nevertheless, heterogeneous integration of dissimilar materials with DNA nanostructures has yet to be explored. We report a DNA origami-encoded strategy for integrating silica-metal heterostructures. Theoretical and experimental studies reveal distinctive mechanisms for the binding and aggregation of silica and metal clusters on protruding double-stranded DNA (dsDNA) strands that are prescribed on the DNA origami template. In particular, the binding energy differences of silica/metal clusters and DNA molecules underlies the accessibilities of dissimilar material areas on DNA origami. By programming the densities and lengths of protruding dsDNA strands on DNA origami, silica and metal materials can be independently deposited at their predefined areas with a high vertical precision of 2 nm. We demonstrate the integration of silica-gold and silica-silver heterostructures with high site addressability. This DNA nanotechnology-based strategy is thus applicable for integrating various types of dissimilar materials, which opens up new routes to bottom-up electronics.

Keywords: DNA metallization; DNA nanotechnology; DNA origami; DNA silicification.

Publication types

Research Support, Non-U.S. Gov't