Complete genome sequence of Clostridium perfringens B20, a bacteriocin-producing pathogen

J Anim Sci Technol. 2021 Nov;63(6):1468-1472. doi: 10.5187/jast.2021.e113. Epub 2021 Nov 30.

Abstract

Clostridium perfringens B20 was isolated from chicken feces collected from a local farm associated with Chung-Ang University (Anseong, Korea). The whole genome of C. perfringens B20 was sequenced using the PacBio RS II platform and assembled de novo. The genome is 2,982,563 bp long and assembled in two contigs. Annotation analyses revealed 2,668 protein-coding sequences, 30 rRNA genes, and 94 tRNA genes, with 28.2% G + C (guanine + cytosine) content. In silico genomic analysis revealed the presence of genes encoding a class IId bacteriocin, lactococcin A, and associated ABC transporter and immunity proteins, as well as a putative bacteriocin gene.

Keywords: Bacteriocin; Clostridium perfringens; Lactococcin A; Pathogen; Virulence; Whole genome sequence.