Complete genome sequence of Bacillus coagulans CACC834 isolated from canine

Jung-Ae Kim; Dae-Hyuk Kim; Yangseon Kim

doi:10.5187/jast.2021.e108

Complete genome sequence of Bacillus coagulans CACC834 isolated from canine

J Anim Sci Technol. 2021 Nov;63(6):1464-1467. doi: 10.5187/jast.2021.e108. Epub 2021 Nov 30.

Authors

Jung-Ae Kim^{1

2}, Dae-Hyuk Kim^{1

3}, Yangseon Kim¹

Affiliations

¹ Department of Research and Development, Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, Korea.
² Department of Bioactive Material Science, Jeonbuk National University, Jeonju 54896, Korea.
³ Department of Molecular Biology, Department of Bioactive Material Science, Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju 54896, Korea.

Abstract

Bacillus coagulans CACC 834 was isolated from canine feces, and its potential probiotic properties were characterized by functional genome analysis. Whole-genome sequencing of B. coagulans CACC 834 was performed using the PacBio RSII platforms. The complete genome assembly consisted of one circular chromosome (3.1 Mb) with guanine (G) + cytosine (C) content of 47.1%. Annotation revealed 3,181 protein-coding sequences (CDSs), 30 rRNAs, and 83 tRNAs. Gene associated 11% of the genes were involved in replication, recombination, and repair. We also annotated various stress-related, acid resistance, bile salt resistance and adhesion-related domains in this strain, which likely provide support in exerting probiotic action by survival under gastrointestinal tract. These results add to our comprehensive understanding of B. coagulans and suggest potential mammal-related industrial applications.

Keywords: Bacillus coagulans; Canine; Whole-genome sequencing.