Immobilized arginine/tryptophan-rich cyclic dodecapeptide on reduced graphene oxide anchored with manganese dioxide for microbial biofilm eradication

Nadia A Samak; Mohamed S Selim; Zhifeng Hao; Jianmin Xing

doi:10.1016/j.jhazmat.2021.128035

Immobilized arginine/tryptophan-rich cyclic dodecapeptide on reduced graphene oxide anchored with manganese dioxide for microbial biofilm eradication

J Hazard Mater. 2022 Mar 15:426:128035. doi: 10.1016/j.jhazmat.2021.128035. Epub 2021 Dec 8.

Authors

Nadia A Samak¹, Mohamed S Selim², Zhifeng Hao³, Jianmin Xing⁴

Affiliations

¹ CAS Key Laboratory of Green Process and Engineering & State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; Environmental Microbiology and Biotechnology, Aquatic Microbiology, University of Duisburg-Essen, 4141 Essen, Germany; Processes Design and Development Department, Egyptian Petroleum Research Institute, Nasr City 11727, Cairo, Egypt; College of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, PR China.
² Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China; Petroleum Application Department, Egyptian Petroleum Research Institute, Nasr City 11727, Cairo, Egypt.
³ Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China. Electronic address: haozf@gdut.edu.cn.
⁴ CAS Key Laboratory of Green Process and Engineering & State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; College of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, PR China; Chemistry and Chemical Engineering Guangdong Laboratory, Shantou 515031, PR China. Electronic address: jmxing@ipe.ac.cn.

PMID: 34954434
DOI: 10.1016/j.jhazmat.2021.128035

Abstract

To avoid the accumulation of bacterial biofilms in water pipelines, it is critical to develop potent antimicrobial agents with good ability to reduce extracellular polymeric substances (EPS). In this study, cyclic dodecapeptides were synthesized, and different mutations for increasing the ratio of arginine (Arg) and tryptophan (Trp) were introduced. Separately, the synthesized dodecapeptides were immobilized on a reduced graphene oxide nanocomposite anchored with a hierarchical β-MnO₂ (RGO/β-MnO₂) hybrid. With a minimum inhibitory concentration of 0.97 g/mL, the immobilized Arg-Trp rich antimicrobial peptides (AMP) on RGO/MnO₂ nanocomposite, Cdp-4/RGO/MnO₂, showed superior efficacy against multidrug-resistant Pseudomonas aeruginosa ATCC 15692 (P. aeruginosa) planktonic cells. The immobilized Cdp-4/RGO/β-MnO₂ also eradicated the mature biofilm by 99% with a minimum inhibitory concentration value of 62.5 µg/mL with significant reduction of EPS. These characteristics allow the use of the immobilized Arg-Trp rich AMP as a promising antimicrobial agent against microbial biofilms, present in water distribution systems.

Keywords: Antimicrobial peptides; Arginine-tryptophan-rich dodecapeptides; Biofilms; Pseudomonas aeruginosa; Water distribution systems.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Antimicrobial Peptides
Arginine
Biofilms
Graphite
Manganese Compounds*
Microbial Sensitivity Tests
Oxides*
Pseudomonas aeruginosa
Tryptophan

Substances

Anti-Bacterial Agents
Antimicrobial Peptides
Manganese Compounds
Oxides
graphene oxide
Graphite
Tryptophan
Arginine
manganese dioxide