Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system

Shuai Zhang; Tianwei Guo; Yuanxiu Song; Mengqi Jiang; Youxu Jiang; Tao Dong; Siyao Zhang; Hong Wang; Xiaotong Hou

doi:10.1016/j.scr.2021.102631

Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system

Stem Cell Res. 2022 Mar:59:102631. doi: 10.1016/j.scr.2021.102631. Epub 2021 Dec 20.

Authors

Shuai Zhang¹, Tianwei Guo², Yuanxiu Song³, Mengqi Jiang⁴, Youxu Jiang², Tao Dong⁵, Siyao Zhang², Hong Wang¹, Xiaotong Hou⁶

Affiliations

¹ Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
² Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing 100029, China.
³ Department of Cardiology, Peking University Third Hospital, Beijing 100191,China.
⁴ Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
⁵ Histology and Embryology Section, Basic Medicine School, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China.
⁶ Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. Electronic address: xt.hou@ccmu.edu.cn.

PMID: 34953326
DOI: 10.1016/j.scr.2021.102631

Abstract

S100A1 is a calcium-binding protein involved in myocardial contractility,which possesses a high affinity for calcium. Several studies have demonstrated that S100A1 is a protector against myocardial injury. In this study, we have generated a homozygous S100A1 knockout (S100A1-KO) human embryonic stem cell (hESC) line by the CRISPR/Cas9 editing system. This S100A1-KO hESC line maintained normal morphology, pluripotency and karyotype, which can differentiate into three germ layers in vivo.