Establishment of human embryonic stem cell (SKLRMe001-A)carrying Azoospermic factor c (AZFc) deletions

Chuyu Li; Lingbo Cai; Juan Dong; Song Ning; Jing Zhou; Huimin Wu; Yugui Cui; Lianju Qin; Xiaoyu Yang

doi:10.1016/j.scr.2021.102625

Establishment of human embryonic stem cell (SKLRMe001-A)carrying Azoospermic factor c (AZFc) deletions

Stem Cell Res. 2022 Mar:59:102625. doi: 10.1016/j.scr.2021.102625. Epub 2021 Dec 18.

Authors

Chuyu Li¹, Lingbo Cai¹, Juan Dong¹, Song Ning¹, Jing Zhou¹, Huimin Wu¹, Yugui Cui¹, Lianju Qin², Xiaoyu Yang³

Affiliations

¹ State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, 210029 Nanjing, Jiangsu, China.
² State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, 210029 Nanjing, Jiangsu, China. Electronic address: ljqin@njmu.edu.cn.
³ State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, 210029 Nanjing, Jiangsu, China. Electronic address: yxy1921@163.com.

PMID: 34952435
DOI: 10.1016/j.scr.2021.102625

Abstract

The absence of azoospermia factor c (AZFc) is a common molecular cause of sperm failure. In men with non-obstructive azoospermia or severe oligospermia, the incidence of AZFc is around 10%. The AZFc region is located at the far end of the Yqll chromosome which has three non-overlapping sub-regions with a high frequency of deletion. Now, we generated a human embryonic stem cell line (SKLRMe001-A) that carries a deleted AZFc gene on the Y chromosome. The ESC line maintains a stem cell-like morphology, pluripotency, and has a normal karyotype. The cells can also differentiate into all three germ layers in vivo.