Single and combined toxicity effects of nanoplastics and bisphenol F on submerged the macrophyte Hydrilla verticillata

Abstract

Nano- and microplastics pose severe risks to the ecological environment. Nanoplastics (NPs) not only directly affect aquatic organisms, but also adsorb to other pollutants, resulting in compound pollution. Bisphenol F (BPF), an endocrine-disrupting chemical, is increasingly replacing bisphenol A (BPA) and is therefore widely distributed in the environment. In this study, the toxic effects of polystyrene nanoplastics (PS-NPs) and BPF and their combined exposure on the submerged macrophytes Hydrilla verticillata (H. verticillata) and leaf biofilms, were investigated. Results showed that 10 mg/L PS-NPs and combined exposure to 10 mg/L PS-NPs and 10 mg/L BPF significantly decreased the relative growth rate and chlorophyll content of H. verticillata, whereas BPF exposure alone had no impact on the growth and the contents of photosynthetic pigments in H. verticillata. Individual and combined exposure to PS-NPs and BPF can trigger antioxidant responses such as increased activities of superoxide dismutase, peroxidase, and malondialdehyde, as well as higher levels of glutathione S-transferase and glutathione and decreased catalase activity. The results of the scanning electron microscopy (SEM) showed that the nanoplastics particles were adsorbed on the surface of plant leaves, explaining their toxic effects, whereas BPF increases the sorption of PS-NPs on the surface of H. verticillata, potentially leading to PS-NPs enrichment in the food chain. The diversity and richness of the microbial community were altered by exposure to PS-NPs and BPF individually and in combination. The current study is the first to assess the effects of PS-NPs and BPF exposure on the growth, physiological characteristics, and leaf biofilm properties of submerged macrophytes.

Keywords: Antioxidant responses; Bisphenol F; Microbial community; Plant growth; Polystyrene nanoplastics; Scanning electron microscopy.