Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (Triticum aestivum L.)

Shuai Tian; Minghu Zhang; Jinghui Li; Shaozhe Wen; Chan Bi; Huanhuan Zhao; Chaoxiong Wei; Zelin Chen; Jiazheng Yu; Xintian Shi; Rongqi Liang; Chaojie Xie; Baoyun Li; Qixin Sun; Yufeng Zhang; Mingshan You

doi:10.3389/fpls.2021.747775

Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (Triticum aestivum L.)

Front Plant Sci. 2021 Dec 7:12:747775. doi: 10.3389/fpls.2021.747775. eCollection 2021.

Authors

Shuai Tian¹, Minghu Zhang¹, Jinghui Li², Shaozhe Wen¹, Chan Bi¹, Huanhuan Zhao¹, Chaoxiong Wei¹, Zelin Chen¹, Jiazheng Yu¹, Xintian Shi¹, Rongqi Liang¹, Chaojie Xie¹, Baoyun Li¹, Qixin Sun^{1

3}, Yufeng Zhang¹, Mingshan You¹

Affiliations

¹ State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, The Ministry of Education, Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China.
² Wheat Center, Henan Institute of Science and Technology, Henan Provincial Key Laboratory of Hybrid Wheat, Xinxiang, China.
³ National Plant Gene Research Centre, Beijing, China.

Abstract

Sodium dodecyl sulfate-sedimentation volume is an important index to evaluate the gluten strength of common wheat and is closely related to baking quality. In this study, a total of 15 quantitative trait locus (QTL) for sodium dodecyl sulfate (SDS)-sedimentation volume (SSV) were identified by using a high-density genetic map including 2,474 single-nucleotide polymorphism (SNP) markers, which was constructed with a doubled haploid (DH) population derived from the cross between Non-gda3753 (ND3753) and Liangxing99 (LX99). Importantly, four environmentally stable QTLs were detected on chromosomes 1A, 2D, and 5D, respectively. Among them, the one with the largest effect was identified on chromosome 1A (designated as QSsv.cau-1A.1) explaining up to 39.67% of the phenotypic variance. Subsequently, QSsv.cau-1A.1 was dissected into two QTLs named as QSsv.cau-1A.1.1 and QSsv.cau-1A.1.2 by saturating the genetic linkage map of the chromosome 1A. Interestedly, favorable alleles of these two loci were from different parents. Due to the favorable allele of QSsv.cau-1A.1.1 was from the high-value parents ND3753 and revealed higher genetic effect, which explained 25.07% of the phenotypic variation, mapping of this locus was conducted by using BC₃F₁ and BC₃F₂ populations. By comparing the CS reference sequence, the physical interval of QSsv.cau-1A.1.1 was delimited into 14.9 Mb, with 89 putative high-confidence annotated genes. SSVs of different recombinants between QSsv.cau-1A.1.1 and QSsv.cau-1A.1 detected from DH and BC₃F₂ populations showed that these two loci had an obvious additive effect, of which the combination of two favorable loci had the high SSV, whereas recombinants with unfavorable loci had the lowest. These results provide further insight into the genetic basis of SSV and QSsv.cau-1A.1.1 will be an ideal target for positional cloning and wheat breeding programs.

Keywords: SDS-sedimentation volume; major QTL; mapping; quality; wheat.