Objective: To investigate the effect of trending up-regulation LncRNA CASC11 which is differentially expressed during the malignant transformation of human bronchial epithelial cells(16 HBE) induced by glycidyl methacrylate(GMA).
Methods: After 16 HBE cells were repeatedly exposed to low concentration GMA(8 μg/mL), the 10 th, 20 th and 30 th passage cells of the GMA group and the DMSO solvent control group were collected. High throughput LncRNA microarray were used to screen the differentially expressed LncRNAs at different stages. Short Time-series Expression Miner and bioinformatics tool RPISeq were used to screen and predict the potential targets of specific LncRNA. Real-time fluorescent quantitative PCR(qPCR) and Western blot were used to detect the relative expression of specific LncRNA and the content of its target protein respectively.
Results: The specific differential expression of LncRNA CASC11 in the process of GMA-induced malignant transformation of 16 HBE cells showed a trend of up-regulation. Compared with the control cells in the same period, the P10, P20 and P30 generation cells were down-regulated by 1.64 times, up-regulated by 2.01 times, and up-regulated by 2.66 times, respectively. Western blot showed that the levels of cyclin-dependent kinase 1(CDK1), cyclinB1 and cyclinB2 in P10, P20 and P30 cells after exposure were lower than those in DMSO control group during the same period, which was consistent with the microarray results.
Conclusion: The differential expression of LncRNA CASC11 in the process of GMA-induced 16 HBE cell malignant transformation was up-regulated trendingly. It is speculated that it may block or delay cell cycle progression by inhibiting CDK1.
Keywords: LncRNA CASC11; cyclin-dependent kinase 1; glycidyl methacrylate; malignant transformation.