Properties of GABAergic Neurons Containing Calcium-Permeable Kainate and AMPA-Receptors

Valery Petrovich Zinchenko; Artem Mikhailovich Kosenkov; Sergei Gennadevich Gaidin; Alexander Igorevich Sergeev; Ludmila Petrovna Dolgacheva; Sultan Tuleukhanovich Tuleukhanov

doi:10.3390/life11121309

Properties of GABAergic Neurons Containing Calcium-Permeable Kainate and AMPA-Receptors

Life (Basel). 2021 Nov 27;11(12):1309. doi: 10.3390/life11121309.

Authors

Valery Petrovich Zinchenko¹, Artem Mikhailovich Kosenkov¹, Sergei Gennadevich Gaidin¹, Alexander Igorevich Sergeev¹, Ludmila Petrovna Dolgacheva¹, Sultan Tuleukhanovich Tuleukhanov²

Affiliations

¹ Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Institute of Cell Biophysics of the Russian Academy of Sciences, 142290 Pushchino, Russia.
² Laboratory of Biophysics, Chronobiology and Biomedicine, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.

Abstract

Calcium-permeable kainate and AMPA receptors (CP-KARs and CP-AMPARs), as well as NMDARs, play a pivotal role in plasticity and in regulating neurotransmitter release. Here we visualized in the mature hippocampal neuroglial cultures the neurons expressing CP-AMPARs and CP-KARs. These neurons were visualized by a characteristic fast sustained [Ca²⁺]_i increase in response to the agonist of these receptors, domoic acid (DoA), and a selective agonist of GluK1-containing KARs, ATPA. Neurons from both subpopulations are GABAergic. The subpopulation of neurons expressing CP-AMPARs includes a larger percentage of calbindin-positive neurons (39.4 ± 6.0%) than the subpopulation of neurons expressing CP-KARs (14.2 ± 7.5% of CB⁺ neurons). In addition, we have shown for the first time that NH₄Cl-induced depolarization faster induces an [Ca²⁺]_i elevation in GABAergic neurons expressing CP-KARs and CP-AMPARs than in most glutamatergic neurons. CP-AMPARs antagonist, NASPM, increased the amplitude of the DoA-induced Ca²⁺ response in GABAergic neurons expressing CP-KARs, indicating that neurons expressing CP-AMPARs innervate GABAergic neurons expressing CP-KARs. We assume that CP-KARs in inhibitory neurons are involved in the mechanism of outstripping GABA release upon hyperexcitation.

Keywords: Ca2+-permeable AMPA receptors; Ca2+-permeable kainate receptors; GABAergic neurons; GluA2 subunit; GluK1 subunit; spontaneous synchronous activity.

Abstract

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