A Novel Antibacterial Titanium Modification with a Sustained Release of Pac-525

Yuzhu He; Yuanyuan Li; Enjun Zuo; Songling Chai; Xiang Ren; Tao Fei; Guowu Ma; Xiumei Wang; Huiying Liu

doi:10.3390/nano11123306

A Novel Antibacterial Titanium Modification with a Sustained Release of Pac-525

Nanomaterials (Basel). 2021 Dec 6;11(12):3306. doi: 10.3390/nano11123306.

Authors

Yuzhu He^{1

2}, Yuanyuan Li^{1

2}, Enjun Zuo¹, Songling Chai¹, Xiang Ren¹, Tao Fei¹, Guowu Ma¹, Xiumei Wang², Huiying Liu¹

Affiliations

¹ School of Stomatology, Dalian Medical University, Dalian 116044, China.
² State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

Abstract

For the benefit of antibacterial Ti on orthopedic and dental implants, a bioactive coating (Pac@PLGA MS/HA coated Ti) was deposited on the surface of pure titanium (Ti), which included two layers: an acid-alkali heat pretreated biomimetic mineralization layer and an electrosprayed Poly (D,L-lactide-co- glycolic acid) (PLGA) microsphere layer as a sustained-release system. Hydroxyapatite (HA) in mineralization layer was primarily prepared on the Ti followed by the antibacterial coating of Pac-525 loaded by PLGA microspheres. After observing the antimicrobial peptides distributed uniformly on the titanium surface, the release assay showed that the release of Pac-525 from Pac@PLGA MS/HA coated Ti provided a large initial burst followed by a slow release at a flat rate. Pac@PLGA MS/HA coated Ti exhibited a strong cytotoxicity to both Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus). In addition, Pac@PLGA MS/HA coated Ti did not affect the growth and adhesion of the osteoblast-like cell line, MC3T3-E1. These data suggested that a bionic mineralized composite coating with long-term antimicrobial activity was successfully prepared.

Keywords: Pac-525; acid–alkali heat pretreated mineralization; electrospray; titanium modification.