hnRNP Q and hnRNP A1 Regulate the Translation of Cofilin in Response to Transient Oxygen-Glucose Deprivation in Hippocampal Neurons

Cells. 2021 Dec 17;10(12):3567. doi: 10.3390/cells10123567.

Abstract

Protein aggregates of cofilin and actin have been found in neurons under oxygen-glucose deprivation. However, the regulatory mechanism behind the expression of Cfl1 during oxygen-glucose deprivation remains unclear. Here, we found that heterogeneous nuclear ribonucleoproteins (hnRNP) Q and hnRNP A1 regulate the translation of Cfl1 mRNA, and formation of cofilin-actin aggregates. The interaction between hnRNP A1 and Cfl1 mRNA was interrupted by hnRNP Q under normal conditions, while the changes in the expression and localization of hnRNP Q and hnRNP A1 increased such interaction, as did the translation of Cfl1 mRNA under oxygen-glucose deprived conditions. These findings reveal a new translational regulatory mechanism of Cfl1 mRNA in hippocampal neurons under oxygen-glucose deprivation.

Keywords: RNA-binding proteins; cofilin–actin aggregates; mRNA translation; neurodegeneration; oxygen–glucose deprivation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Depolymerizing Factors / genetics
  • Actin Depolymerizing Factors / metabolism*
  • Animals
  • Brain Ischemia / pathology
  • Cells, Cultured
  • Disease Models, Animal
  • Glucose / deficiency*
  • Heterogeneous Nuclear Ribonucleoprotein A1 / metabolism*
  • Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
  • Hippocampus / pathology*
  • Mice
  • Neurons / metabolism*
  • Oxygen / metabolism*
  • Protein Binding
  • Protein Biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • Actin Depolymerizing Factors
  • Heterogeneous Nuclear Ribonucleoprotein A1
  • Heterogeneous-Nuclear Ribonucleoproteins
  • RNA, Messenger
  • Syncrip protein, mouse
  • Glucose
  • Oxygen