Physicochemical and Biological Study of 99mTc and 68Ga Radiolabelled Ciprofloxacin and Evaluation of [99mTc]Tc-CIP as Potential Diagnostic Radiopharmaceutical for Diabetic Foot Syndrome Imaging

Przemysław Koźmiński; Weronika Gawęda; Magdalena Rzewuska; Agata Kopatys; Szymon Kujda; Marta K Dudek; Paweł Krzysztof Halik; Leszek Królicki; Ewa Gniazdowska

doi:10.3390/tomography7040070

Physicochemical and Biological Study of ^99mTc and ⁶⁸Ga Radiolabelled Ciprofloxacin and Evaluation of [^99mTc]Tc-CIP as Potential Diagnostic Radiopharmaceutical for Diabetic Foot Syndrome Imaging

Tomography. 2021 Dec 1;7(4):829-842. doi: 10.3390/tomography7040070.

Authors

Przemysław Koźmiński¹, Weronika Gawęda¹, Magdalena Rzewuska², Agata Kopatys³, Szymon Kujda³, Marta K Dudek⁴, Paweł Krzysztof Halik¹, Leszek Królicki³, Ewa Gniazdowska¹

Affiliations

¹ Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.
² Institute of Veterinary Medicine, Department of Preclinical Sciences, Division of Microbiology, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland.
³ Department of Nuclear Medicine, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw, Poland.
⁴ Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland.

Abstract

This paper presents the application of ciprofloxacin as a biologically active molecule (vector) for delivering diagnostic radiopharmaceuticals to the sites of bacterial infection. Ciprofloxacin-based radioconjugates containing technetium-99m or gallium-68 radionuclides were synthesised, and their physicochemical (stability, lipophilicity) and biological (binding study to Staphylococcus aureus and Pseudomonas aeruginosa) properties were investigated. Both the tested radiopreparations met the requirements for radiopharmaceuticals, and technetium-99m-labelled ciprofloxacin turned out to be a good radiotracer for the tomography of diabetic foot syndrome using SPECT.

Keywords: bacterial infection; ciprofloxacin; diabetic foot; gallium-68; radiopharmaceutical; technetium-99m.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ciprofloxacin
Diabetes Mellitus*
Diabetic Foot* / diagnostic imaging
Gallium Radioisotopes
Humans
Radiopharmaceuticals
Staphylococcal Infections* / diagnostic imaging

Substances

Gallium Radioisotopes
Radiopharmaceuticals
Ciprofloxacin