Low C/N wastewater results from a wide range of factors that significantly harm the environment. They include insufficient carbon sources, low denitrification efficiency, and NH4+-N concentrations in low C/N wastewater that are too high to be treated. In this research, the membrane biofilm reactor and hydrogen-based membrane biofilm reactor (MBR-MBfR) were optimized and regulated under different operating parameters: the simulated domestic sewage with low C/N was domesticated and the domestic sewage was then denitrified. The results of the MBR-MBfR experiments indicated that a C/N ratio of two was suitable for NH4+-N, NO2--N, NO3--N, and chemical oxygen demand (COD) removal in partial nitrification-denitrification (PN-D) and hydrogen autotrophic denitrification for further treatment. The steady state for domestic wastewater was reached when the MBR-MBfR in the experimental conditions of HRT = 15 h, SRT = 20 d, 0.04 Mpa for H2 pressure in MBfR, 0.4-0.8 mg/L DO in MBR, MLSS = 2500 mg/L(MBR) and 2800 mg/L(MBfR), and effluent concentrations of NH4+-N, NO3--N, and NO2--N were 4.3 ± 0.5, 1.95 ± 0.04, and 2.05 ± 0.15 mg/L, respectively. High-throughput sequencing results revealed the following: (1) The genus Nitrosomonas as the ammonia oxidizing bacteria (AOB) and Denitratisoma as potential denitrifiers were simultaneously enriched in the MBR; (2) at the genus level, Meiothermus,Lentimicrobium, Thauera,Hydrogenophaga, and Desulfotomaculum played a dominant role in leading to NO3--N and NO2--N removal in the MBfR.
Keywords: MBR-MBfR; hydrogen autotrophic denitrification; partial nitrification-denitrification.