Mass Spectrometry for O-GlcNAcylation

Ruoting Yin; Xin Wang; Cheng Li; Yuhan Gou; Xuecheng Ma; Yongzhao Liu; Jianfang Peng; Chao Wang; Ying Zhang

doi:10.3389/fchem.2021.737093

Mass Spectrometry for O-GlcNAcylation

Front Chem. 2021 Dec 6:9:737093. doi: 10.3389/fchem.2021.737093. eCollection 2021.

Authors

Ruoting Yin¹, Xin Wang¹, Cheng Li¹, Yuhan Gou¹, Xuecheng Ma¹, Yongzhao Liu¹, Jianfang Peng¹, Chao Wang¹, Ying Zhang¹

Affiliation

¹ Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, China.

Abstract

O-linked β-N-acetylglucosamine modification (O-GlcNAcylation) at proteins with low-abundance expression level and species diversity, shows important roles in plenty of biological processes. O-GlcNAcylations with abnormal expression levels are associated with many diseases. Systematically profiling of O-GlcNAcylation at qualitative or quantitative level is vital for their function understanding. Recently, the combination of affinity enrichment, metabolic labeling or chemical tagging with mass spectrometry (MS) have made significant contributions to structure-function mechanism elucidating of O-GlcNAcylations in organisms. Herein, this review provides a comprehensive update of MS-based methodologies for quali-quantitative characterization of O-GlcNAcylation.

Keywords: O-GlcNAc; O-GlcNAcylated proteins; O-GlcNAcylation; isotope labeling; mass spectrometry; quali-quantitative charactering.

Publication types

Review