[Does alpha-tocopherol interact with the active center of cytochrome P-450 in liver microsomes?]

Biull Eksp Biol Med. 1987 Mar;103(3):304-6.
[Article in Russian]

Abstract

The interaction of alpha-tocopherol (alpha-T) and its synthetic derivative 2,2,5,7,8-pentamethyl-6-hydroxy-chroman (PMC) with cytochrome P-450 system was studied in the rat liver microsomes. Spectral differentiations of type I, increase of NADPH oxidation rate and inhibition of 7-ethoxycoumarin deethylase in microsomes were observed only in the presence of PMC. The results obtained suggest that unlike alpha-T, PMC is effectively bound and metabolized by cytochrome P-450.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • 7-Alkoxycoumarin O-Dealkylase
  • Animals
  • Binding Sites
  • Biotransformation
  • Catalysis
  • Chromans / metabolism
  • Cytochrome P-450 Enzyme System / metabolism*
  • Drug Interactions
  • Kinetics
  • Microsomes, Liver / enzymology*
  • NAD / metabolism
  • Oxygenases / metabolism
  • Rats
  • Structure-Activity Relationship
  • Substrate Specificity
  • Vitamin E / metabolism*

Substances

  • Chromans
  • NAD
  • Vitamin E
  • Cytochrome P-450 Enzyme System
  • 2,2,5,7,8-pentamethyl-1-hydroxychroman
  • Oxygenases
  • 7-Alkoxycoumarin O-Dealkylase