Abstract
The interaction of alpha-tocopherol (alpha-T) and its synthetic derivative 2,2,5,7,8-pentamethyl-6-hydroxy-chroman (PMC) with cytochrome P-450 system was studied in the rat liver microsomes. Spectral differentiations of type I, increase of NADPH oxidation rate and inhibition of 7-ethoxycoumarin deethylase in microsomes were observed only in the presence of PMC. The results obtained suggest that unlike alpha-T, PMC is effectively bound and metabolized by cytochrome P-450.
Publication types
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Comparative Study
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English Abstract
MeSH terms
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7-Alkoxycoumarin O-Dealkylase
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Animals
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Binding Sites
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Biotransformation
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Catalysis
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Chromans / metabolism
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Cytochrome P-450 Enzyme System / metabolism*
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Drug Interactions
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Kinetics
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Microsomes, Liver / enzymology*
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NAD / metabolism
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Oxygenases / metabolism
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Rats
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Structure-Activity Relationship
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Substrate Specificity
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Vitamin E / metabolism*
Substances
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Chromans
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NAD
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Vitamin E
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Cytochrome P-450 Enzyme System
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2,2,5,7,8-pentamethyl-1-hydroxychroman
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Oxygenases
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7-Alkoxycoumarin O-Dealkylase