In this study, the antioxidant status, oxidant status, antimicrobial activity, and antiproliferative activity of wild mushroom, Laeticutis cristata, were determined. Total antioxidant status (TAS) and total oxidant status (TOS) were determined using the Rel Assay TAS kit and the Rel Assay TOS kit, respectively. Antibacterial and antifungal activities were determined using the modified agar dilution method. An antiproliferative effect was determined by the MTT cell viability assay against lung cancer cell line A549. L. cristata demonstrated significant antioxidant potential, with a TAS value of 3.623 ± 0.250, TOS of 27.476 ± 0.314, and oxidative stress index of 0.765 ± 0.051. In addition, its antifungal activity was high (25 µg/mL). L. cristata was determined to have a strong antiproliferative effect against A549. As a result, it is thought that L. cristata can be used as a natural material in pharmacological designs.