Analysis of antioxidant activity on cocktail honey products as female pre-conception supplements

Abstract

Objective: Cocktail honey is derived from a mixture of honey (trigona sp.), bee bread, and homogeneous royal jelly. The material has a phenolic content rich in antioxidants that are beneficial for women's reproductive health, especially for pre-conception, because it can suppress the content of free radicals in the body. Antioxidants are useful to overcome oxidative damage due to free radicals in the body that prevent various diseases from increasing fertility during pre-conception.

Method: This study used the DPPH (2,2-diphenyl-1-picrylhydrazyl) test method using UV-vis spectrophotometry to express the value of free radical reduction activity as IC₅₀ (inhibitory concentration) values.

Results: The DPPH test on cocktail honey products obtained an average yield of 4577.7μg/mL, which was included in the product category was very weak in the antioxidant activity content.

Conclusion: The content contained in the honey cocktail contains weak bioactive content by assessing the antioxidant content using DPPH. The difference in the results of antioxidant activity tests using DPPH is caused by the test method and the conditions used in processing, homogeneous ingredients, solvent volume, extraction time, temperature, and pressure in product management.

Keywords: Antioxidant; Cocktail honey; Nutrition; Pre-conception supplement; Reproductive health.