Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system

Stem Cell Res. 2022 Jan:58:102627. doi: 10.1016/j.scr.2021.102627. Epub 2021 Dec 13.

Abstract

Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems / genetics
  • Heterozygote
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Homozygote
  • Humans
  • Induced Pluripotent Stem Cells* / metabolism
  • Myocytes, Cardiac / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Homeodomain Proteins
  • IRX5 protein, human
  • Transcription Factors