Activatable fluorogenic probe for accurate imaging of ulcerative colitis hypoxia in vivo

Mingrui Li; Yong Zhang; Xiaojun Ren; Wenchao Niu; Qing Yuan; Kai Cao; Jinchao Zhang; Xueyun Gao; Dongdong Su

doi:10.1039/d1cc06577g

Activatable fluorogenic probe for accurate imaging of ulcerative colitis hypoxia in vivo

Chem Commun (Camb). 2022 Jan 18;58(6):819-822. doi: 10.1039/d1cc06577g.

Authors

Mingrui Li¹, Yong Zhang¹, Xiaojun Ren¹, Wenchao Niu¹, Qing Yuan¹, Kai Cao¹, Jinchao Zhang², Xueyun Gao¹, Dongdong Su¹

Affiliations

¹ Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing, 100124, P. R. China. xjren@bjut.edu.cn.
² College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding, 071002, P. R. China.

PMID: 34928281
DOI: 10.1039/d1cc06577g

Abstract

A simple but efficient fluorogenic probe is reported for accurate imaging of ulcerative colitis via hypoxia detection. The hypoxia produced by ulcerative colitis can lead to the upregulation of nitroreductase (NTR). NB-NO2 provides a unique response to NTR, enabling accurate imaging of Dextran sulphate sodium (DSS)-induced ulcerative colitis in vivo.

MeSH terms

A549 Cells
Animals
Colitis, Ulcerative / chemically induced
Colitis, Ulcerative / diagnostic imaging*
Dextran Sulfate / toxicity
Diagnostic Imaging / methods*
Disease Models, Animal
Fluorescent Dyes / chemistry*
Humans
Hypoxia
Mice
Microscopy, Fluorescence
Nitric Oxide / chemistry
Nitric Oxide / metabolism
Nitroreductases / metabolism
Up-Regulation

Substances

Fluorescent Dyes
Nitric Oxide
Dextran Sulfate
Nitroreductases