Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

Jana Pekarkova; Imrich Gablech; Tatiana Fialova; Ondrej Bilek; Zdenka Fohlerova

doi:10.3389/fbioe.2021.782799

Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

Front Bioeng Biotechnol. 2021 Dec 3:9:782799. doi: 10.3389/fbioe.2021.782799. eCollection 2021.

Authors

Jana Pekarkova^{1

2}, Imrich Gablech^{1

2}, Tatiana Fialova³, Ondrej Bilek⁴, Zdenka Fohlerova^{1

2

5}

Affiliations

¹ Central European Institute of Technology, Brno University of Technology, Brno, Czechia.
² Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno, University of Technology, Brno, Czechia.
³ Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czechia.
⁴ Institute of Environmental Technology, VŠB-Technical University of Ostrava, Ostrava, Czechia.
⁵ Department of Biochemistry, Faculty of Medicine, Masaryk University, Brno, Czechia.

Abstract

Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C-coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.

Keywords: antimicrobial; biocompatibility; micropillars; parylene-C; selenium nanoparticles.