Developmental morphology of the turkey pineal gland in histological images and 3D models

Abstract

The histological structure of the avian pineal gland during embryonic life has so far only been studied in chickens. It is known that the pineal organs of hatched chickens and turkeys differ significantly from each other based on their morphology and physiology. The aim of the present study was to investigate the histological structure of the embryonic pineal gland of domestic turkeys. The study was performed on turkey embryos aged 4-28 days. Along with histological analyses, three-dimensional (3D) images of the pineal glands from embryos aged 6-28 days were also obtained. In four-day-old embryos [embryonic day (ED) 4], primary evagination of the pineal gland from the neuroectoderm of the diencephalon was observed. On ED 6, the evagination formed a pineal recess with a thick and folded wall. In the next embryonic stages, the pineal recess was lengthened to the pineal canal, with the lumen opening to the third ventricle. The connection of the pineal lumen with the ventricular lumen was observed in all studied embryos. The first cellular rosettes without the lumen separated from the wall of the pineal recess occurred on ED 6. Several small and round follicles containing their own lumens were visible on ED 8. On ED 10, the pineal parenchyma was composed mainly of small, round follicles. The first oval follicles appeared on ED 12 and branched follicles appeared on ED 16. In some embryos at different stages, follicles formed from secondary evaginations of the diencephalon epithelium were observed. The turkey pineal organ maintained the follicular type of parenchyma without solid cellular aggregates throughout embryonic life. The pineal follicles originated from: 1) rosettes arising from the wall of the pineal canal (from ED 6); 2) an accessory evagination occurring in the neuroectoderm anteriorly and posteriorly to the pineal canal end (from ED 6); 3) direct development in the walls of larger follicles and detaching from them in a manner similar to the budding process (from ED 14); and 4) fusion of smaller follicles into branched ones. The pineal capsule started to develop on ED 6, first as a vascularization and later as a thin mesenchymal outline around the apical part, then at the dorsal and at the end the ventral part of the pineal gland. The pineal stroma was composed of mesenchymal tissue consisting of abundant in cells and blood vessels. The first evagination of the choroid plexus in the diencephalon was observed on ED 8. The attachment of the pineal gland to the dura mater first occurred on ED 16. Finally, the pineal gland of ED 28 embryos consisted of a wide proximal part attached to the dura mater and a narrow distal part that extended into the pineal stalk, which extended to the intercommissural region of the diencephalon. The present study revealed the occurrence of significant morphological differences in the developing embryonic pineal gland of turkeys compared with chickens.

Keywords: 3D; Development; Embryos; Histology; Pineal gland; Turkey.