Effect of an extrafibrillar dentin demineralization strategy on the durability of the resin-dentin bond

Ming Xing Li; Lian Duan; Mei Ling Chen; Fu Cong Tian; Bai Ping Fu

doi:10.1016/j.jmbbm.2021.105038

Effect of an extrafibrillar dentin demineralization strategy on the durability of the resin-dentin bond

J Mech Behav Biomed Mater. 2022 Feb:126:105038. doi: 10.1016/j.jmbbm.2021.105038. Epub 2021 Dec 11.

Authors

Ming Xing Li¹, Lian Duan², Mei Ling Chen³, Fu Cong Tian⁴, Bai Ping Fu⁵

Affiliations

¹ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, China.
² College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, China.
³ Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
⁴ The Dental College of Georgia, Augusta University, Augusta, GA, USA.
⁵ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, China. Electronic address: fbp@zju.edu.cn.

PMID: 34923366
DOI: 10.1016/j.jmbbm.2021.105038

Abstract

Objectives: This study aimed to evaluate the potential of the extrafibrillar dentin demineralization strategy on the long-term dentin bond strength of an etch-and-rinse adhesive.

Methods: A water-soluble glycol chitosan-EDTA (GCE), a chelating conditioner, was synthesized and subjected to size-exclusion dialysis to obtain molecules >40 kDa. The conjugation of EDTA to glycol chitosan was analyzed by Fourier transform infrared (FTIR) spectroscopy. Mid-coronal dentin surfaces of 80 teeth were either acid-etched with 35% phosphoric acid or conditioned with 25 mg/mL GCE (n = 40) and thoroughly water-sprayed before applying the etch-and-rinse adhesive Adper Single Bond Plus and placing Z250 composite resin (3 M Oral Care; St Paul, MN, USA). Resin-bonded specimens were prepared into beams with a cross-sectional area of about 0.9 mm² vertically through the resin-dentin interfaces before the microtensile bond strengths (MTBS) were determined immediately or after 3, 6, or 12 months of water storage. The resin-dentin interfaces were analyzed using transmission electron microscopy (TEM). The MTBS data were analyzed using two-way ANOVA followed by the LSD post-hoc multiple comparisons (P < 0.05).

Results: FTIR spectra showed that EDTA was successfully conjugated to glycol chitosan. The phosphoric acid-etching group and GCE-conditioning group showed similar bond strength values after 24 h of water storage. The bond strength of the phosphoric acid-etching group after 12-month water aging was significantly reduced from 51.61 ± 3.30 MPa to 38.57 ± 4.81 MPa, while the bond strength of the GCE-conditioning group was not significantly reduced from 50.28 ± 3.62 MPa to 46.40 ± 4.71 MPa.The degradation of the hybrid layer could be detected in the phosphoric acid-etching group after 12 months of water aging, but not in the GCE-conditioning group.

Conclusion: The extrafibrillar dentin demineralization strategy using GCE conditioner could defy the hybrid layer degradation of the dentin bond after 12 months of water aging and enhance the dentin bond durability of the etch-and-rinse adhesive Adper Single Bond Plus.

Keywords: Dentin bond durability; Extrafibrillar dentin demineralization; Glycol chitosan-EDTA; Hybrid layer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Composite Resins
Dental Bonding*
Dentin
Dentin-Bonding Agents*
Materials Testing
Microscopy, Electron, Scanning
Resin Cements
Tensile Strength

Substances

Composite Resins
Dentin-Bonding Agents
Resin Cements