Heat shock factor 1 suppression induces spindle abnormalities and sensitizes cells to antimitotic drugs

Hsiao-Hui Kuo; Zhi-Rou Su; Jing-Yuan Chuang; Ling-Huei Yih

doi:10.1186/s13008-021-00075-8

Heat shock factor 1 suppression induces spindle abnormalities and sensitizes cells to antimitotic drugs

Cell Div. 2021 Dec 18;16(1):8. doi: 10.1186/s13008-021-00075-8.

Authors

Hsiao-Hui Kuo¹, Zhi-Rou Su^{1

2}, Jing-Yuan Chuang², Ling-Huei Yih³

Affiliations

¹ Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 115, Taiwan.
² Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
³ Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 115, Taiwan. lhyih@gate.sinica.edu.tw.

Abstract

Background: Heat shock factor 1 (HSF1) is the master regulator of the heat shock response and supports malignant cell transformation. Recent work has shown that HSF1 can access the promoters of heat shock proteins (HSPs) and allow HSP expression during mitosis. It also acts as a mitotic regulator, controlling chromosome segregation. In this study, we investigated whether the transactivation activity of HSF1 is required for the assembly of mitotic spindles.

Results: Our results showed that phosphorylation of HSF1 at serine 326 (S326) and its transactivation activity were increased during mitosis. Inhibition of the transactivation activity of HSF1 by KRIBB11 or CCT251263 during mitosis significantly increased the proportion of mitotic cells with abnormal spindles. It also hampered the reassembly of spindle microtubules after nocodazole treatment and washout by impeding the formation of chromosomal microtubule asters. Depletion of HSF1 led to defects in mitotic spindle assembly, subsequently attenuating cell proliferation and anchorage-independent cell growth (AIG). These HSF1 depletion-induced effects could be rescued by ectopically expressing wild-type HSF1 or a constitutively active mutant (∆202-316, caHSF1) but not the S326A or dominant negative (∆361-529, dnHSF1) mutants. In addition, overexpression of HSP70 partially reduced HSF1 depletion-induced spindle abnormalities. These results indicate that HSF1 may support cell proliferation and AIG by maintaining spindle integrity through its transactivation activity. Furthermore, inhibition of HSF1 transactivation activity by KRIBB11 or CCT251236 can enhance diverse anti-mitosis drug-induced spindle defects and cell death.

Conclusions: The increased transactivation activity of HSF1 during mitosis appears to be required for accurate assembly of mitotic spindles, thereby supporting cell viability and probably AIG. In addition, inhibition of the transactivation activity of HSF1 may enhance the mitotic errors and cell death induced by anti-mitosis drugs.

Keywords: Cell death; HSF1; Spindle assembly.