Confocal laser scanning microscopy evaluation of roots subjected to activation protocol in endodontic microsurgery

Aust Endod J. 2022 Apr;48(1):77-81. doi: 10.1111/aej.12598. Epub 2021 Dec 17.

Abstract

This study evaluated the penetration of the irrigant subject to activation after performing retro-preparation in endodontic microsurgery. Forty mandibular premolars were prepared and filled. Subsequently, 1 mm from the root apex was cut using a multi-blade bur and the retro-preparation was performed. In group 1, the retro-cavity was cleaned with 2 mL of saline and then with 2 mL of 5.25% NaOCl gel mixed with 0.1% Rhodamine B. In group 2, the retro-cavity was cleaned with 2 mL of saline, 17% EDTA gel was activated ultrasonically for 30 s and 5.25% NaOCl gel was mixed with 0.1% Rhodamine B and activated for 30 s. After 7 days, the roots were cut at 1mm from the apex and the slices were evaluated with confocal laser scanning microscopy. The samples of group 2 showed statistically better results. The activation protocol proposed resulted in enhanced irrigant penetration when compared to the traditional.

Keywords: dentinal tubules penetration; endodontic microsurgery; irrigant penetration; retro-preparation; ultrasonic.

MeSH terms

  • Dental Pulp Cavity
  • Dentin
  • Microscopy, Confocal
  • Microsurgery
  • Root Canal Irrigants* / therapeutic use
  • Root Canal Preparation* / methods
  • Sodium Hypochlorite

Substances

  • Root Canal Irrigants
  • Sodium Hypochlorite