Isolation of Normal and Cancer-Associated Fibroblasts

Katarzyna Zawieracz; Mark A Eckert

doi:10.1007/978-1-0716-1956-8_10

Isolation of Normal and Cancer-Associated Fibroblasts

Methods Mol Biol. 2022:2424:155-165. doi: 10.1007/978-1-0716-1956-8_10.

Authors

Katarzyna Zawieracz¹, Mark A Eckert²

Affiliations

¹ Department of Obstetrics and Gynecology/Section of Gynecologic Oncology, University of Chicago, Chicago, IL, USA.
² Department of Obstetrics and Gynecology/Section of Gynecologic Oncology, University of Chicago, Chicago, IL, USA. meckert@bsd.uchicago.edu.

PMID: 34918293
DOI: 10.1007/978-1-0716-1956-8_10

Abstract

Cancer-associated fibroblasts (CAFs) play important roles in regulating tumor progression, metastasis, and response to therapies. Accurately modeling the interplay between cancer cells and the tumor microenvironment (TME) requires the use of primary cells from patient samples. Here we describe methods for the isolation of both primary CAFs and fibroblasts from omental tissue using a combination of mechanical dissociation and enzymatic digestion. Primary cells can be used for functional and mechanistic studies and may be safely cryopreserved.

Keywords: Cancer; Cancer-associated fibroblasts; Fibroblasts; Isolation; Mesothelial cells; Metastasis; Omentum; Ovarian cancer; Primary cells; Stroma; Tumor microenvironment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cancer-Associated Fibroblasts*
Fibroblasts
Humans
Neoplasms*
Omentum
Tumor Microenvironment