Upcycling of brewers' spent grains via solid-state fermentation for the production of protein hydrolysates with antioxidant and techno-functional properties

Yi Ling Chin; Kong Fei Chai; Wei Ning Chen

doi:10.1016/j.fochx.2021.100184

Upcycling of brewers' spent grains via solid-state fermentation for the production of protein hydrolysates with antioxidant and techno-functional properties

Food Chem X. 2021 Dec 7:13:100184. doi: 10.1016/j.fochx.2021.100184. eCollection 2022 Mar 30.

Authors

Yi Ling Chin^{1

2}, Kong Fei Chai², Wei Ning Chen^{1

2}

Affiliations

¹ School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore City 637459, Singapore.
² Food Science and Technology Programme, Nanyang Technological University, Singapore City 637459, Singapore.

Abstract

Brewers' spent grains (BSG) were fermented with Rhizopus oligosporus and up to 15% of original protein was hydrolysed. Fermented BSG was then subjected to an ethanolic-alkali extraction and isolated fractions contained 61-66% protein. An evaluation of functional properties suggested that fermented extracts presented superior emulsifying abilities (15-34 m²/g of activity and 16-42 min of stability), foaming properties (16-30% capacity and 7-14% stability), and water/oil binding capacities (0.41 g/g and 0.24 g/g, respectively). They also showed significantly higher ABTS inhibition and stronger reducing power than unfermented ones, indicating that fermented BSG protein extract had greater antioxidant activities. No cytotoxic effect was detected in the range of 2-10 mg/mL. When applied in a mayonnaise formulation, fermented hydrolysates demonstrated better emulsion stability in terms of creaming, microstructure and viscosity. Thus, fermented BSG protein is a potential plant-based emulsifier for food, pharmaceutical and cosmetic applications.

Keywords: Brewers’ spent grains; Food waste valorisation; Microbial fermentation; Plant-based emulsifier; Protein hydrolysate.