The epidemiology and control of Toxocara canis in puppies

Regan Schwartz; Satesh Bidaisee; Paul J Fields; Maxine L A Macpherson; Calum N L Macpherson

doi:10.1016/j.parepi.2021.e00232

The epidemiology and control of Toxocara canis in puppies

Parasite Epidemiol Control. 2021 Dec 8:16:e00232. doi: 10.1016/j.parepi.2021.e00232. eCollection 2022 Feb.

Authors

Regan Schwartz^{1

2

3}, Satesh Bidaisee^{1

2

4

3}, Paul J Fields^{2

3}, Maxine L A Macpherson^{1

2

3}, Calum N L Macpherson^{1

2

4

3}

Affiliations

¹ School of Veterinary Medicine, St. George's University, Grenada.
² School of Graduate Studies, St. George's University, Grenada.
³ Windward Islands Research and Education Foundation, Grenada.
⁴ School of Medicine, St. George's University, Grenada.

Abstract

Toxocara canis is a helminth zoonosis that is estimated to infect more than 100 million dogs and 1 billion people, mostly in the tropics. Humans can be infected by accidentally ingesting embryonated T. canis eggs from the environment or occasionally after ingesting L₃ larvae from paratenic hosts. This study investigated the importance of vertical transmission and the role of puppies in the epidemiology of T. canis through the examination of fecal samples from dogs less than one year of age in Grenada, West Indies, a small island tropical developing country. Samples were stored at 4 °C or in 10% formalin until microscopic examination for helminth eggs or using a rapid antigen test for the presence of protozoan species. A knowledge, attitudes and practices study was completed among dog owners, physicians and veterinary students. Of 306 dogs less than one year of age, 147 (48%) were found to have T. canis eggs. Vertical transmission was indicated by the proportion of infected dogs increasing from 50% at two weeks of age (from in utero transmission) to 70% by 12 weeks (in utero and lactogenic transmission). After 12 weeks the positivity rate dropped rapidly with no dogs over 40 weeks of age being infected. As T. canis eggs were found in puppy feces at two weeks of age, initial treatment of puppies should begin earlier, at twelve days post-partum, than currently recommended to prevent shedding of eggs. Perhaps even more importantly, treatment of pregnant dogs, preventing vertical transmission, would have a major impact on the control of T. canis infection. Knowledge of T. canis and other zoonotic helminths such as Ancylostoma caninum was found to be low among dog owners, physicians and veterinary students. None of the dog owners treated their dogs for helminths, all were unaware of the risk of zoonoses, and only 9% picked up dog feces. Efforts to prevent vertical transmission and to increase awareness and knowledge of these zoonoses could result in reducing their public health impact.

Keywords: Ancylostoma caninum; Control; Epidemiology; Puppies; Toxocara canis; Vertical transmission.