Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

Chuangjia Huang; Xiaoling Guan; Hui Lin; Lu Liang; Yingling Miao; Yueheng Wu; Huiqiong Bao; Xiaodan Wu; Ao Shen; Minyan Wei; Jionghua Huang

doi:10.3389/fbioe.2021.762956

Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

Front Bioeng Biotechnol. 2021 Nov 30:9:762956. doi: 10.3389/fbioe.2021.762956. eCollection 2021.

Authors

Chuangjia Huang^{1

2}, Xiaoling Guan^{1

2}, Hui Lin³, Lu Liang^{1

2}, Yingling Miao^{1

2}, Yueheng Wu⁴, Huiqiong Bao⁴, Xiaodan Wu^{1

2}, Ao Shen^{1

2}, Minyan Wei^{1

2}, Jionghua Huang¹

Affiliations

¹ Department of Cardiology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
² Key Laboratory of Molecular Target and Clinical Pharmacology and the State and NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
³ Department of Oncology, Guangdong Provincial Hospital of Integrated Traditional Chinese and Western Medicine, Foshan, China.
⁴ School of Medicine, Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences, South China University of Technology, Guangzhou, China.

Abstract

Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye approved by the Food and Drug Administration (FDA), has been extensively used as a photoacoustic (PA) probe for PA imaging. However, its practical application is limited by poor photostability in water, rapid body clearance, and non-specificity. Herein, we fabricated a novel biomimetic nanoprobe by coating ICG-loaded mesoporous silica nanoparticles with the cancer cell membrane (namely, CMI) for PA imaging. This probe exhibited good dispersion, large loading efficiency, good biocompatibility, and homologous targeting ability to Hela cells in vitro. Furthermore, the in vivo and ex vivo PA imaging on Hela tumor-bearing nude mice demonstrated that CMI could accumulate in tumor tissue and display a superior PA imaging efficacy compared with free ICG. All these results demonstrated that CMI might be a promising contrast agent for PA imaging of cervical carcinoma.

Keywords: ICG; cell membrane coating; cervical carcinoma; mesoporous silica nanoparticles; photoacoustic imaging.