Generation of CpG-Recoded Zika Virus Vaccine Candidates

Ivan Trus; Daniel Udenze; Uladzimir Karniychuk

doi:10.1007/978-1-0716-1884-4_14

Generation of CpG-Recoded Zika Virus Vaccine Candidates

Methods Mol Biol. 2022:2410:289-302. doi: 10.1007/978-1-0716-1884-4_14.

Authors

Ivan Trus¹, Daniel Udenze¹, Uladzimir Karniychuk²

Affiliations

¹ Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK, Canada.
² Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK, Canada. u.karniychuk@usask.ca.

PMID: 34914053
DOI: 10.1007/978-1-0716-1884-4_14

Abstract

Experimental increase of cytosine-phosphate-guanine (CpG) dinucleotides in an RNA virus genome impairs infection. Beneficially, this weak infection may lead to robust antiviral host immunity providing a cutting-edge approach for vaccines. For example, we have recently demonstrated that recoded Zika virus variants with the increased CpG content showed considerable attenuated infection phenotypes and protection against lethal challenge in mice. Here, we describe the workflow for the design and generation of CpG-recoded Zika virus vaccine candidates. The workflow can be adapted for other viruses.

Keywords: CpG dinucleotide; Recoding; Stock; Vaccine; Zika virus.

MeSH terms

Animals
Antibodies, Viral
Mice
Phosphates
Viral Vaccines
Zika Virus Infection* / prevention & control
Zika Virus* / immunology

Substances

Antibodies, Viral
Phosphates
Viral Vaccines