Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development

Nishanthi Mathiyalagan; Sebastian Dworkin

doi:10.1007/978-1-0716-1847-9_2

Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development

Methods Mol Biol. 2022:2403:19-32. doi: 10.1007/978-1-0716-1847-9_2.

Authors

Nishanthi Mathiyalagan¹, Sebastian Dworkin²

Affiliations

¹ Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC, Australia.
² La Trobe University, Bundoora, VIC, Australia. s.dworkin@latrobe.edu.au.

PMID: 34913113
DOI: 10.1007/978-1-0716-1847-9_2

Abstract

Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5 days postfertilization.

Keywords: Craniofacial; Gene expression; In-situ hybridization; Neural crest; Riboprobe; Zebrafish.

MeSH terms

Animals
Embryo, Nonmammalian / metabolism
Gene Expression Regulation, Developmental
In Situ Hybridization
Neural Crest / metabolism
Skull
Zebrafish Proteins / metabolism
Zebrafish* / genetics
Zebrafish* / metabolism

Substances

Zebrafish Proteins