Towards CRISPR powered electrochemical sensing for smart diagnostics

Poyye Dsouza Priya Swetha; Jospeh Sonia; Kannan Sapna; K Sudhakara Prasad

doi:10.1016/j.coelec.2021.100829

Towards CRISPR powered electrochemical sensing for smart diagnostics

Curr Opin Electrochem. 2021 Dec:30:100829. doi: 10.1016/j.coelec.2021.100829. Epub 2021 Aug 12.

Authors

Poyye Dsouza Priya Swetha¹, Jospeh Sonia¹, Kannan Sapna¹, K Sudhakara Prasad^{1

2}

Affiliations

¹ Nanomaterial Research Laboratory (NMRL), Nano Division, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, 575 018, India.
² Centre for Nutrition Studies, Yenepoya (Deemed to be University), Deralakatte, Mangalore, 575 018, India.

Abstract

Even though global health has been steadily improved, the global disease burden associated with communicable and non-communicable diseases extensively increased healthcare expenditure. The present COVID-19 pandemic scenario has again ascertained the importance of clinical diagnostics as a basis to make life-saving decisions. In this context, there is a need for developing next-generation integrated smart real-time responsive biosensors with high selectivity and sensitivity. The emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas biosensing systems has shown remarkable potential for developing next-generation biosensors. CRISPR/Cas integrated electrochemical biosensors (E-CRISPR) stands out with excellent properties. In this opinionated review, we illustrate the rapidly evolving applications for E-CRISPR-integrated detection systems towards biosensing and the future scope associated with E-CRISPR based diagnostics.

Keywords: Biosensing; CRISPR/Cas; E-CRISPR; Electrochemical; Point-of-care.

Publication types

Review