Isolation of CD31+ Bone Marrow Endothelial Cells (BMECs) from Mice

Alhaji Osman Smith; Seyram Yao Adzraku; Wen Ju; Jianlin Qiao; Kailin Xu; Lingyu Zeng

doi:10.21769/BioProtoc.4227

Isolation of CD31⁺ Bone Marrow Endothelial Cells (BMECs) from Mice

Bio Protoc. 2021 Nov 20;11(22):e4227. doi: 10.21769/BioProtoc.4227.

Authors

Alhaji Osman Smith^{1

2}, Seyram Yao Adzraku^{1

2}, Wen Ju^{1

2

3}, Jianlin Qiao^{1

2

3}, Kailin Xu^{1

2

3}, Lingyu Zeng^{1

2

3}

Affiliations

¹ Blood Diseases Institute, Xuzhou Medical University, Xuzhou 221002, China.
² Key Laboratory of the Bone Marrow Stem Cell Transplantation, Xuzhou 221002, China.
³ Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China.

Abstract

In the bone marrow microenvironment, endothelial cells (ECs) play a pivotal role in regulating the production of both growth and inhibiting factors. They are held together by adherence molecules that interact with hematopoietic progenitor cells. The study of ECs in the hematopoietic stem cell niche is limited due to the lack of efficient protocols for isolation. In this protocol, we developed a two-step approach to extract bone marrow endothelial cells (BMECs) to unlock the challenges researchers face in understanding the function of the endothelial vascular niche in in-vitro studies.

Keywords: Bone marrow endothelial cells (BMECs); Endothelial cells (ECs); Endothelial-vascular niche; Extraction; Isolation; Mouse bone marrow.