Sodium channel mutations (T929I and F1534S) found in pyrethroid-resistant strains of the cigarette beetle, Lasioderma serricorne (Coleoptera: Anobiidae)

Naoto Fukazawa; Ryota Takahashi; Hinako Matsuda; Yuya Mikawa; Toshiyuki Suzuki; Tomohiro Suzuki; Shoji Sonoda

doi:10.1584/jpestics.D21-033

Sodium channel mutations (T929I and F1534S) found in pyrethroid-resistant strains of the cigarette beetle, Lasioderma serricorne (Coleoptera: Anobiidae)

J Pestic Sci. 2021 Nov 20;46(4):360-365. doi: 10.1584/jpestics.D21-033.

Authors

Naoto Fukazawa¹, Ryota Takahashi¹, Hinako Matsuda², Yuya Mikawa², Toshiyuki Suzuki³, Tomohiro Suzuki³, Shoji Sonoda²

Affiliations

¹ Leaf Tobacco Research Center, Japan Tobacco Inc., Oyama, Tochigi 323-0808, Japan.
² School of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321-8505, Japan.
³ Center for Bioscience Research and Education, Utsunomiya University,Utsunomiya, Tochigi 321-8505, Japan.

Abstract

RNA-seq data analysis of cigarette beetle (Lasioderma serricorne) strains having different sensitivities to pyrethroids identified sodium channel mutations in strains showing pyrethroid resistance: the T929I and F1534S mutations. These results suggest that reduced sensitivity of the sodium channel confers the pyrethroid resistance of L. serricorne. Results also showed that the F1534S mutation mostly occurred concurrently with the T929I mutation. The functional relation between both mutations for pyrethroid resistance is discussed.

Keywords: PCR-RFLP; insecticide resistance; stored products; target insensitivity; tobacco beetle.

© Pesticide Science Society of Japan 2021. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License (https://creativecommons.org/licenses/by-nc-nd/4.0/).