Effect of BMP6 on the proliferation and apoptosis of chicken chondrocytes induced by thiram

Yuxiang Lu; Hengyong Xu; Yuru Jiang; Dan Li; Zhi Hu; Chaoyang Yan; Huadong Yin; Diyan Li; Xiaoling Zhao; Yao Zhang; Yaofu Tian; Qing Zhu; Yan Wang

doi:10.1016/j.rvsc.2021.12.002

Effect of BMP6 on the proliferation and apoptosis of chicken chondrocytes induced by thiram

Res Vet Sci. 2021 Dec 7:142:101-109. doi: 10.1016/j.rvsc.2021.12.002. Online ahead of print.

Authors

Yuxiang Lu¹, Hengyong Xu¹, Yuru Jiang¹, Dan Li¹, Zhi Hu¹, Chaoyang Yan¹, Huadong Yin¹, Diyan Li¹, Xiaoling Zhao¹, Yao Zhang¹, Yaofu Tian¹, Qing Zhu², Yan Wang³

Affiliations

¹ Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, 211# Huiming Street, Wenjiang district Chengdu, 611130, China.
² Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, 211# Huiming Street, Wenjiang district Chengdu, 611130, China. Electronic address: zhuqingsicau@163.com.
³ Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, 211# Huiming Street, Wenjiang district Chengdu, 611130, China. Electronic address: as519723614@163.com.

PMID: 34906792
DOI: 10.1016/j.rvsc.2021.12.002

Abstract

The development of skeleton system is a complex biological process and be regulated by many transcription factors. Previous studies have shown that BMP6 is involved in skeleton development and other cells transforming to chondrocytes, but it is still not known whether do something to tibial dyschondroplasia (TD) broilers chondrocytes. In this study, RT-PCR revealed that the expression level of BMP6 in TD broiler chondrocytes at 7 days age was significantly decreased compared with normal group (P < 0.05). CCK-8 and EdU assay showed that the proliferation of cells transfected with interference BMP6 was significantly decreased compared with control siRNA, while cell proliferation was significantly increased after overexpression of BMP6. Meanwhile, the proportion of G₀/G₁ phase cells was significantly increased and the proportion of G₂/M phase cells was significantly decreased after interference of BMP6 for 48 h in TD chicken chondrocytes (P < 0.05). In addition, flow cytometry analysis exhibited that interference BMP6 significantly increased apoptosis rate and necrotizing rate of cells. In conclusion, these results suggest that BMP6 plays a positive role in the growth and development of TD broiler chondrocytes. Our findings reveal a new target for TD prevention in broiler chickens.

Keywords: Apoptosis; BMP6; Chicken; Proliferation; Tibial dyschondroplasia.