Abstract
A 35S-labeled 48-base synthetic oligonucleotide complementary to a portion of the rat corticotropin-releasing factor (CRF) mRNA was used for in situ hybridization histochemistry. CRF-synthesizing cells were located in the paraventricular nucleus of the hypothalamus. These cells were observed in the medial parvocellular subdivision where there was a 90% increase in the amount of CRF mRNA per unit volume after adrenalectomy.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adrenalectomy*
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Animals
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Autoradiography
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Corticotropin-Releasing Hormone / genetics*
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Histocytochemistry
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Male
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Nucleic Acid Hybridization
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Paraventricular Hypothalamic Nucleus / metabolism*
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RNA, Messenger / biosynthesis*
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Rats
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Rats, Brattleboro
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Rats, Inbred Strains
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Sulfur Radioisotopes
Substances
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RNA, Messenger
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Sulfur Radioisotopes
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Corticotropin-Releasing Hormone