Quantitative in situ hybridization histochemistry reveals increased levels of corticotropin-releasing factor mRNA after adrenalectomy in rats

Neurosci Lett. 1986 Oct 8;70(2):198-203. doi: 10.1016/0304-3940(86)90463-5.

Abstract

A 35S-labeled 48-base synthetic oligonucleotide complementary to a portion of the rat corticotropin-releasing factor (CRF) mRNA was used for in situ hybridization histochemistry. CRF-synthesizing cells were located in the paraventricular nucleus of the hypothalamus. These cells were observed in the medial parvocellular subdivision where there was a 90% increase in the amount of CRF mRNA per unit volume after adrenalectomy.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenalectomy*
  • Animals
  • Autoradiography
  • Corticotropin-Releasing Hormone / genetics*
  • Histocytochemistry
  • Male
  • Nucleic Acid Hybridization
  • Paraventricular Hypothalamic Nucleus / metabolism*
  • RNA, Messenger / biosynthesis*
  • Rats
  • Rats, Brattleboro
  • Rats, Inbred Strains
  • Sulfur Radioisotopes

Substances

  • RNA, Messenger
  • Sulfur Radioisotopes
  • Corticotropin-Releasing Hormone