RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants

Jing-Ze Zou; De-Shui Liu; Xin Tong; Xiao-Peng Zhang; Xian-Bing Wang

doi:10.1007/978-1-0716-1835-6_27

RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants

Methods Mol Biol. 2022:2400:283-296. doi: 10.1007/978-1-0716-1835-6_27.

Authors

Jing-Ze Zou¹, De-Shui Liu¹, Xin Tong¹, Xiao-Peng Zhang¹, Xian-Bing Wang²

Affiliations

¹ State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
² State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, China. wangxianbing@cau.edu.cn.

PMID: 34905211
DOI: 10.1007/978-1-0716-1835-6_27

Abstract

RNA in situ hybridization, a histological technique derived from Southern blotting and northern blotting, has been an important approach in biology studies for many years. In the studies of virus-plant interactions, RNA in situ hybridization provides a direct visualization of viral RNA in host plants. Here, we provide a detailed protocol for viral RNA in situ hybridization that has been successfully used to detect Cucumber mosaic virus genome (CMV) RNAs in shoots of N. benthamiana plants.

Keywords: Cucumber mosaic virus; Digoxigenin (DIG) labelling; Nicotiana benthamiana; RNA in situ hybridization; Shoot apical meristems.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cucumovirus* / genetics
In Situ Hybridization
Nicotiana / genetics
Plant Diseases
RNA, Viral / genetics

Substances

RNA, Viral