A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus

Ji-Hui Qiao; Qiang Gao; Ying Zang; Xiao-Dong Fang; Xian-Bing Wang

doi:10.1007/978-1-0716-1835-6_16

A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus

Methods Mol Biol. 2022:2400:163-170. doi: 10.1007/978-1-0716-1835-6_16.

Authors

Ji-Hui Qiao¹, Qiang Gao¹, Ying Zang¹, Xiao-Dong Fang¹, Xian-Bing Wang²

Affiliations

¹ State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
² State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, China. wangxianbing@cau.edu.cn.

PMID: 34905200
DOI: 10.1007/978-1-0716-1835-6_16

Abstract

In recent years, plant virus-based vectors have been widely applied to express heterologous proteins for genomic studies and commercial production. Among these versatile RNA viral vectors, the barley yellow striate mosaic virus (BYSMV)-based expression vector system has outstanding capability to express large and multiple heterologous proteins. Here we describe a detailed protocol for expression of heterologous proteins using BYSMV expression systems in monocot plants and insects.

Keywords: Barley yellow striate mosaic virus; Monocot plants; RNA viral vectors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Edible Grain / virology
Genetic Vectors / genetics
Genomics
Insecta / genetics
Plant Viruses*
Rhabdoviridae* / genetics