Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses

Rongrong He; Mark A Bernards; Aiming Wang

doi:10.1007/978-1-0716-1835-6_12

Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses

Methods Mol Biol. 2022:2400:115-123. doi: 10.1007/978-1-0716-1835-6_12.

Authors

Rongrong He^{1

2}, Mark A Bernards², Aiming Wang³

Affiliations

¹ London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
² Department of Biology, The University of Western Ontario, London, ON, Canada.
³ London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada. aiming.wang@canada.ca.

PMID: 34905196
DOI: 10.1007/978-1-0716-1835-6_12

Abstract

In plants, plasmodesmata (PD) are plasmamembrane-lined pores that traverse the cell wall to establish cytoplasmic and endomembrane continuity between neighboring cells. As intercellular channels, PD play pivotal roles in plant growth and development, defense responses, and are also co-opted by viruses to spread cell-to-cell to establish systemic infection. Proteomic analyses of PD-enriched fractions may provide critical insights on plasmodesmal biology and PD-mediated virus-host interactions. However, it is difficult to isolate PD from plant tissues as they are firmly embedded in the cell wall. Here, we describe a protocol for the purification of PD from Nicotiana benthamiana leaves for proteomic analysis.

Keywords: Nicotiana benthamiana; Plasmodesmata; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Wall
Nicotiana
Plants
Plasmodesmata*
Proteomics*