Microbial Identification in the Clinical Microbiology Laboratory Using MALDI-TOF-MS

Iván Bloise; M Teresa Corcuera; Julio García-Rodríguez; Jesús Mingorance

doi:10.1007/978-1-0716-1936-0_16

Microbial Identification in the Clinical Microbiology Laboratory Using MALDI-TOF-MS

Methods Mol Biol. 2022:2420:207-216. doi: 10.1007/978-1-0716-1936-0_16.

Authors

Iván Bloise¹, M Teresa Corcuera¹, Julio García-Rodríguez¹, Jesús Mingorance²

Affiliations

¹ Servicio de Microbiología, Hospital Universitario La Paz-IdiPAZ, Madrid, Spain.
² Servicio de Microbiología, Hospital Universitario La Paz-IdiPAZ, Madrid, Spain. jesus.mingorance@idipaz.es.

PMID: 34905176
DOI: 10.1007/978-1-0716-1936-0_16

Abstract

MALDI-TOF mass spectrometry has been applied with huge success to the identification of cultured microorganisms in clinical microbiology laboratories where it has become the reference method because it is simple, fast, and highly reproducible. We describe the different procedures used in the routine for pathogen identification using the Bruker MALDI Biotyper^® system.

Keywords: Biotyper; Clinical microbiology; MALDI-TOF; Pathogens.

MeSH terms

Laboratories
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*