Suggested Cutoff Point for Testosterone by Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) after Stimulation with Recombinant Human Chorionic Gonadotropin

Leticia R de Oliveira; Carlos A Longui; Guilherme Guaragna-Filho; José L da Costa; Rafael Lanaro; Maria I Chiamolera; Maricilda P de Mello; André M Morcillo; Andrea T Maciel-Guerra; Gil Guerra-Junior

doi:10.1159/000519422

Suggested Cutoff Point for Testosterone by Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) after Stimulation with Recombinant Human Chorionic Gonadotropin

Sex Dev. 2022;16(4):266-269. doi: 10.1159/000519422. Epub 2021 Dec 14.

Authors

Leticia R de Oliveira^{1

2}, Carlos A Longui³, Guilherme Guaragna-Filho^{1

4}, José L da Costa^{5

6}, Rafael Lanaro^{5

6}, Maria I Chiamolera⁷, Maricilda P de Mello^{1

8}, André M Morcillo⁹, Andrea T Maciel-Guerra^{1

10}, Gil Guerra-Junior^{1

9}

Affiliations

¹ Interdisciplinary Group for Studies of Sex Determination and Differentiation (GIEDDS), School of Medical Sciences (FCM), State University of Campinas (UNICAMP), Campinas, Brazil.
² Department of Pediatrics, Federal University of Uberlandia (UFU), Uberlandia, Brazil.
³ Pediatric Endocrinology Unit, School of Medical Sciences, Irmandade da Santa Casa de Misericordia de Sao Paulo, Sao Paulo, Brazil.
⁴ Department of Pediatrics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
⁵ School of Pharmaceutical Sciences, UNICAMP, Campinas, Brazil.
⁶ Poison Control Center, FCM, UNICAMP, Campinas, Brazil.
⁷ Fleury Group, Sao Paulo, Brazil.
⁸ Laboratory of Human Molecular Genetics, Center for Molecular Biology and Genetics Engineering (CBMEG), UNICAMP, Campinas, Brazil.
⁹ Department of Pediatrics, FCM, UNICAMP, Campinas, Brazil.
¹⁰ Department of Medical Genetics and Genomic Medicine, FCM, UNICAMP, Campinas, Brazil.

PMID: 34903701
DOI: 10.1159/000519422

Abstract

The human chorionic gonadotropin (hCG) stimulation test that evaluates gonadal steroidogenesis is crucial in the assessment of patients with 46,XY disorders of sex development (DSD). This study aimed to determine a testosterone (T) cutoff level that indicates an adequate testicular function using LC-MS/MS after stimulation with recombinant human chorionic gonadotropin (rhCG) in a single dose. Nineteen prepubertal children with 46,XY DSD and normal T secretion were evaluated. T and dihydrotestosterone (DHT) levels were measured by liquid chromatography technique with tandem mass spectrometry (LC-MS/MS) before and 7 days after rhCG application at 250 µg. We suggest 0.89 ng/mL as the cutoff point for T after rhCG stimulation analyzed by LC-MS/MS.

Keywords: Disorders of sex development; Human chorionic gonadotropin; Testosterone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Child
Chorionic Gonadotropin / pharmacology
Chromatography, Liquid
Humans
Tandem Mass Spectrometry*
Testosterone* / pharmacology

Substances

Testosterone
Chorionic Gonadotropin