Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics

Bingming Chen; Marissa Vavrek; Mark T Cancilla; Vilmos Kertesz

doi:10.1007/978-1-0716-2030-4_12

Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics

Methods Mol Biol. 2022:2437:171-180. doi: 10.1007/978-1-0716-2030-4_12.

Authors

Bingming Chen¹, Marissa Vavrek², Mark T Cancilla², Vilmos Kertesz³

Affiliations

¹ Department of Preclinical Development, Merck & Co., Inc., Kenilworth, NJ, USA. bingming.chen@merck.com.
² Department of Preclinical Development, Merck & Co., Inc., Kenilworth, NJ, USA.
³ Bioanalytical Mass Spectrometry Group, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

PMID: 34902148
DOI: 10.1007/978-1-0716-2030-4_12

Abstract

dropletProbe mass spectrometry is a novel technique for molecular characterization of surfaces. It can be used for rapid ex vivo analysis of therapeutics from thin animal tissue sections and has been shown to improve understanding of a drug's absorption, distribution, metabolism and excretion (ADME) properties. Here, we describe the tissue distribution analysis of diclofenac from a dosed whole-body mouse thin tissue section using a dropletProbe mass spectrometry system.

Keywords: Diclofenac; DropletProbe; Mass spectrometry; Metabolite identification; Tissue distribution; Whole-body analysis.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Diclofenac
Mass Spectrometry*
Mice
Microtomy
Tissue Distribution

Substances

Diclofenac