Extraction and Upconcentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry Imaging

Sanghwan Park; Chang Young Lee

doi:10.1007/978-1-0716-2030-4_11

Extraction and Upconcentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry Imaging

Methods Mol Biol. 2022:2437:159-169. doi: 10.1007/978-1-0716-2030-4_11.

Authors

Sanghwan Park¹, Chang Young Lee²

Affiliations

¹ School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea.
² School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea. cylee@unist.ac.kr.

PMID: 34902147
DOI: 10.1007/978-1-0716-2030-4_11

Abstract

Mass spectrometry imaging (MSI) allows label-free detection of a wide range of biomolecules and simultaneously provides their spatial distributions. In particular, MSI by matrix-assisted laser desorption/ionization mass spectrometry (MALDI) has been widely used in biomolecule analysis. However, quantitation in MALDI-MSI is limited by matrix-deposition heterogeneity, analyte extraction area, and analyte-matrix cocrystallization. In this chapter, a microstructured PDMS stamp is utilized to precisely control the matrix deposition area and the analyte extraction area. We describe here simple steps-including stamp fabrication, matrix application, and data-acquisition guideline-for the quantitative analysis of adsorbed peptides on hydrophobic surfaces.

Keywords: Controlled extraction; Mass spectrometry imaging; Matrix-assisted laser desorption/ionization; Peptides; Quantitation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diagnostic Imaging
Diagnostic Tests, Routine
Peptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

Peptides