MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging

Klaus Dreisewerd; Tanja Bien; Jens Soltwisch

doi:10.1007/978-1-0716-2030-4_2

MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging

Methods Mol Biol. 2022:2437:21-40. doi: 10.1007/978-1-0716-2030-4_2.

Authors

Klaus Dreisewerd¹, Tanja Bien², Jens Soltwisch²

Affiliations

¹ Institute of Hygiene, University of Münster, Münster, Germany. klaus.dreisewerd@uni-muenster.de.
² Institute of Hygiene, University of Münster, Münster, Germany.

PMID: 34902138
DOI: 10.1007/978-1-0716-2030-4_2

Abstract

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) combined with laser-induced postionization for MALDI-2 enables the simultaneous registration of numerous classes of small molecules (e.g., secondary metabolites including sterols) as well as phospholipids, glycolipids, and glycans from tissue sections and from cell cultures with strongly boosted ion yields. Here, we describe methodological aspects that are key for optimizing the analytical sensitivity and spatial resolution of a MALDI-2 imaging experiment. We will include both top-illumination MALDI-2 as well as the recently introduced transmission (t-) mode MALDI-2 approach.

Keywords: Cell cultures; MALDI imaging; MALDI-2; Sample preparation; Transmission-mode MALDI-2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diagnostic Imaging
Glycolipids
Lasers
Phospholipids
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

Glycolipids
Phospholipids